Scientific Program : Scientific Sessions

XIVth International Workshop Molecular and Cellular Biology of Plasminogen Activation

Tuesday, June 4th, 2013

Notre Dame Conference Center (McKenna Hall)
5:00 pm - Registration
6:00 pm - Opening Reception (Harpist - Devon Haupt)
7:30 pm - Opening Comments (Francis J. Castellino)
7:30 pm - Keynote Address - Désire Collen - Serendipity in the Development of Tissuetype Plasminogen Activator
   
Wednesday, June 5th, 2013

McKenna Hall
8:00 am - Registration
8:45 am - Welcoming Address (Robert Bernhard, V. P. Research)
9:00 am - Oral Session 1 – Protease Systems in Inflammation (Chairs: Toni Antalis and Enming Joseph Su)
9:00 am - 01 dot Apo(a) inhibits plasminogen-dependent MMP-9 activation in inflammation
Menggui Huang, Yanqing Gong, Jessica Grondolsky, Nan Zhao, Francis J. Castellino, Victoria A. Ploplis, Jane Hoover-Plow
9:15 am - 02 dot Molecular mechanisms of antiphagocytic activity mediated by plasminogenbinding group A streptococcal M-like protein
Garima Agrahari, Zhong Liang, Victoria A. Ploplis, Francis J. Castellino
9:30 am - 03 dot A role of plasminogen in macrophage foam cell formation
Edward Plow
9:45 am - 04 dot A mannose receptor-mediated collagen degradation pathway by M2
macrophages identified by imaging collagen turnover in vivo
Daniel Madsen, Daniel Leonard, Andrius Masedunskas, Amanda Moyer, Henrik Jürgensen, Diane Peters, Panomwat Amornphimoltham, Arul Selvaraj, Susan Yamada, Lars Engelholm, Niels Behrendt, Kenn Holmbeck, Roberto Weigert, Thomas Bugge
10:00 am - 05 dot Plasminogen regulates macrophage phagocytosis
Riku Das
10:15 am - 06 dot Prostasin is required for matriptase activation during the formation and maintenance of the intestinal epithelial barrier
Marguerite Buzza, Erik Martin, Kathyrn Driesbaugh, Antoine Desilets, Richard Leduc, Toni Antalis
10:30 am - Break
10:45 am - Oral Session 2 – Clinical and Pharmacological Advances in Plasminogen Activation and Fibrinolysis (Chairs: Tetsumei Urano and Claire Booyjzsen)
10:45 am - 07 dot TM5441, a novel PAI-1 antagonist, prevents hypertension and arteriosclerosis in an experimental model for vascular aging
Amanda E Boe, Mesut Eren, Sheila B. Murphy, Christine E. Kamide, Toshio Miyata, and Douglas Vaughan
11:00 am - 08 dot Site-specific PEGlation of human plasminogen activator inhibitor type 2: Fine tuning the pharmacokinetic and biodistribution properties for improved diagnostic and therapeutic potential
Maria Ranson, Vineesh Indira Chandran, Nathaniel Harris, Sergei Lobov, Kara Vine
11:15 am - 09 dot Role of PAI-1 in side effects of radiation therapy: an update
Fabien Milliat
11:30 am - 10 dot Is the phenotype manifested by complete PAI-1 deficiency in human compatible to that in mouse?
Kotomi Nagahashi, Takayuki Iwaki, Katsuhiro Takano, Yukio Ozaki, Naohiro Kanayama, Kazuo Umemura, Tetsumei Urano
11:45 pm - 11 dot Incidence of fibrinolysis measured by thromboelastography in patients with early coagulopathy of Traumatic Brain Injury (CTBI): Implication for therapeutic effectiveness of tranexamic acid (TXA)
Chitta Hanuma, Joseph Capannari, Mark Walsh, Braxton Fritz, Scott Thomas, Michael Chapman, Victoria Ploplis, Deborah Donahue, Megan Maloney, Patrick Davis, Francis Castellino
12:00 pm - 12 dot Engineering allosteric activators of pro-hepatocyte growth factor for met signaling with zymogen activator peptides (ZAPtides) by phage display
Kyle E. Landgraf, Micah Steffek, Clifford Quan, Jeffrey Tom, Christine Yu, Lydia Santell, Henry Maun, Charles Eigenbrot, Robert A. Lazarus
12:15 pm - Lunch and Free time (Business meeting after lunch in auditorium)
2:00 pm - Campus tour (Eck Alumni Hall)
4:00 pm - State-of-the-art talk - Lindsey Miles - Plasminogen Biology: Retrospectives and Prospectives
4:30 pm - Break
4:45 pm - Oral Session 3 – Structure/Function of the Plasminogen System and Related Proteins (Chairs: Nuala Booth and Hongmin Sun)
4:45 pm - 13 dot Structure and function studies on human plasminogen glycoforms
Ruby Law, Tom Caradoc-Davies, Nathan Cowieson, Bernadine Lu, Adam Quek, Anita Horvath, Paul Coughlin, James Whisstock
5:00 pm - 14 dot Latency transition of zebrafish PAI-1: A protein conformational change sterically hindered by an N-linked glycan
Rene Bager, Jesper S. Johansen, Jan K. Jensen, Allan Stensballe, Agnieszka Jendroszek, Hans Peter Sorensen, Peter A. Andreasen
5:15 pm - 15 dot Human tissue kallikreins 3 and 5 can act as plasminogen activator releasing active plasmin
Lucas R. de Souza, Pollyana M. Melo, Thaysa Paschoalin, Adriana K. Carmona, Marcia Kondo, Izaura Y. Hirata, Michael Blaber, Ivarne Tersariol, Joyce Takatsuka, Maria A. Juliano, Luiz Juliano, Roseli A. Gomes, Luciano Puzer
5:30 pm - 16 dot Key residues within core-domain of group A streptococcal plasminogen-binding M-like protein downplays the role of dimerization in plasminogen binding
Sarbani Bhattacharya, Zhong Liang, Victoria A. Ploplis, Francis J. Castellino
5:45 pm 17 dot Identification of a novel, nanobody-induced, mechanism of TAFI inactivation and its in vivo application
Maarten Hendrickx, Monika Zatloukalova, Gholamreza Hassanzadeh-Ghassabeh, Serge Muyldermans, Ann Gils, Paul Declerck
6:00 pm - 18 dot Rezymogenation of active urokinase-type plasminogen activator, induced by an inhibitory antibody
Longguang Jiang, Kenneth A. Botkjaer, Lisbeth M. Andersen, Cai Yuan, Mingdong Huang, Peter Andreasen
7:00 pm - Dinner (Club Naimoli at the Purcell Pavillion) (Steve Gibons – violinist)

 

Thursday, June 6th, 2013

McKenna Hall

8:30 am - State-of-the-art talk - Martina Sanderson-Smith - Understanding the Nexus Between Microbes and Plasminogen in Invasive Pathogens
9:00 am - Oral Session 4 – Regulation of Infection by Components of the Plasminogen System (Chairs: Mark Walker and Shaun Lee)
9:00 am - 19 dot Plasminogen activation, coagulation, and fibrinolysis in primary pneumonicnplague
Adam Caulfield, Lindsay Gielda, Jay Schroeder, Lauren Bellows, Jay Degen, Wyndham Lathem
9:15 am - 20 dot Plasmin acquisition by group A streptococcus protects against C3b-mediated neutrophil phagocytosis
Diane Ly, Jude Taylor, James Tsatsaronis, Mercedes Monteleone, Amanda Skora, Cortny Donald, Tracy Maddocks, Victor Nizet, Nicholas West, Marie Ranson, MarknWalker, Jason McArthur, Martina L. Sanderson-Smith
9:30 am - 21 dot Characterization of streptokinases SK1 and SK2b from group A streptococci
Yueling Zhang, Zhong Liang, Jeffery Mayfield, Kristofor Glinton, Victoria A. Ploplis, Francis J. Castellino
9:45 am - 22 dot Contribution of human plasmin(ogen) to invasive disease propensity of group A streptococci
Mark J. Walker
10:00 am - 23 dot Pharmacological targeting of plasmin prevents inflammatory diseases like septic shock and acute graft-versus-host disease
Aki Sato, Heissig Beate, Koichi Hattori
10:15 am - Break and Group Picture
11:00 am - Oral Session 5 - Proteolytic Processes in Vascular Cell Biology (Chairs: Dudley Strickland and Linda Fredriksson)
11:00 am - 24 dot uPAR's domain 2 regulates single chain urokinase-mediated angiogenesis through β1-integrin and VEGFR2
Alvin Schmaier, Gretchen LaRusch, Alona Merkulova, Fakhri Mahdi, Douglas Cines
11:15 am - 25 dot An urokinase receptor-derived peptide inhibiting VEGF-dependent directional migration and vascular sprouting
Katia Bifulco, Eleonora Liguori, Luigi Mele, Gioconda Di Carluccio, Claudio Arra, Domenica Rea, Maria Teresa Masucci, Maria Patrizia Stopelli, Maria VIncenza Carriero
11:30 am - 26 dot In vivo effects of mutation of tyrosine 573 in the cytoplasmic tail of membranetype 1 matrix metalloproteinase (MT1-MMP)
Paolo Mignatti
11:45 am - 27 dot Real time imaging analysis of secreted-tPA dependent fibrinolytic activity on vascular endothelial cell surface and its enhancement by newly synthesized PAI-1 inhibitor
Hideki Yasui, Yuko Suzuki, Tomasz Brzoska, Hideto Sano, Takafumi Suda, Takashi Dan, Toshio Miyata, Tetsumei Urano
12:00 pm – 28 dot Converging pathways of uPAR up-regulation in cancer and endothelial cells
Anna Laurenzana, Francesca Margheri, Simona Serrati, Nicola Schiavone, Laura Papucci, Lucia Magnelli, Anastasia Chillà, Francesca Bianchini, Lido Calorini, Mario Del Rosso, Gabriella Fibbi
12:15 pm Official Welcome to the 22nd International Congress of Fibrinolysis and Proteolysis (2014) in Marseille, France
Marie-Christine Alessi
12:30 pm - Lunch and Free time
4:00 pm - Manned Poster Session
6:30 pm - Bus pick-up (at hotels and McKenna Hall)
7:00 pm - Workshop Banquet - Studebaker Museum (Music: Cecile Savage Jazz Quartet)
10:00 pm - Return to Hotels
   
Friday, June 7th, 2013

McKenna Hall
8:30 am - Oral Session 6 – Effects of the Plasminogen System in Cell Signaling (Maria Patrizia Stoppelli and Takayuki Iwaki)
8:30 am - 29 dot Ligand-specific co-receptor recruitment determines the signaling activity of LRP1 in response to tissue-type plasminogen activator and myelin-associated glycoprotein
Elisabetta Mantuano, Travis L. Stiles, Dustin Hicks, Steven L. Gonias
8:45 am - 30 dot Novel role of the urokinase receptor in regulating the tumor suppressor PTEN
Matthias Unseld, Anastasia Chilla, Clemens Pausz, Johannes Breuss, Marina Poettler, Gernot Schabbauer, Gerald Prager
9:00 am - 31 dot Urokinase receptor mediates osteogenic differentiation of mesenchymal stem cells via the complement C5a receptor and NFκB
Parnian Kalbasianaraki, Margret Patecki, Sergey Tkachuk, Hermann Haller, Inna Dumler
9:15 am - 32 dot Regulation of hepatic stellate cells through LRP1: a signaling role for t-PA in liver
Liang-I Kang, Kumicko Isse, Anne Orr, William C. Bowen, Anthony J. Demetris, Selen C. Muratoglu, Dudley K. Strickland, George K. Michalopoulos, Wendy M. Mars
9:30 am - 33 dot Requirement of specific sphingolipids to uPAR and EGFR signaling
Maria Patrizia Stoppelli, Paola Franco, Anna De Vincenzo, Maria Nappo, Stefania Belli
9:45 am - 34 dot uPAR bridges the mesenchymal and amoeboid style of cell migration
Francesca Margheri, Anna Laurenzana, Simona Serrati, Alessio Biagioni, Nicola Schiavone, Laura Papucci, Lucia Magnelli, Cristina Luciani, Anastasia Chillà, Paola Chiarugi, Maria Letizia Taddei, Gabriella Fibbi, Mario Del Rosso
10:00 am – Break
10:30 am - Oral Session 7 – Tissue plasminogen activator in neurobiology (Chairs: Robert Medcalf and Patrick Sheets)
10:30 am - 35 dot Neuroserpin/tPA regulate vascular integrity and seizure severity in the murine CNS
Linda Fredriksson, Tamara S. Stevenson, Enming Joseph Su, Margaret Ragsdale, Kris Mann, Daniel Lawrence
10:45 am - 36 dot Is tPA involved in the entorhinal cortice-dependent spatial learning processes?
Marie Hebert, Denis Vivien, Veronique Agin
11:00 am - 37 dot The benefit of compartmentalizing tissue plasminogen activator during stroke
EJ Su, Linda Fredriksson, David Bushart, Margaret Ragsdale, Daniel A. Lawrence
11:15 am - 38 dot Synergistic inhibitors halt brain and systemic hemorrhage after tissue plasminogen activator treatment for ischemic stroke
Yi Zhang, Aiilyan Houng, Guy Reed
11:30 am - 39 dot Single-chain tPA versus two-chain tPA in the control of NMDA receptors signaling
Thomas Bertrand, Jérôme Parcq, Flavie Lesept, Axel Montagne, Jean-Marie Billard, Yannick Hommet, Jialing Wu, Manuel Yepes, Roger Lijnen, Patrick Dutar, Denis Vivien
11:45 am - 40 dot The contribution of alpha 2-antiplasmin to tissue plasminogen activator therapy for stroke: Effects on neuronal cell death, brain swelling, breakdown of the blood brain barrier and mortality
Dong Wang, Aiilyan Houng, Guy Reed
12:00 pm - Lunch and Free time
2:00 pm - Campus tour (Eck Alumni Hall)

Jordan Science Hall
4:30 pm - State-of-the-art talk - Denis Vivien - The "Ying-Yang" of Tissue-type Plasminogen Activator in the Central Nervous System
5:00pm - Break
5:15 pm - Oral Session 8 – The Plasminogen System in Regulating Cell Function (Chairs:
Steven Gonias and Kamlesh Gupta)
5:30 pm - 41 dot Tissue-type plasminogen activator is an extracellular mediator of Purkinje cell damage and altered gait
Elisa Cops, Maithili Sashindranath, Maria Daglas, Kieran Short, Candida da Fonseca Pereira, Terence Pang, Ian Smyth, Anthony Hannan, Andre Samson, Robert Medcalf
5:45 pm - 42 dot Tissue-type plasminogen activator mediates the detection and adaptation to metabolic stress in the central nervous system
Manuel Yepes, Fang Wu, Andrew D. Nicholson, Woldeab B. Haile, Enrique Torre
6:00 pm - 43 dot TAFI is a growth-regulating factor of proliferating hepatocytes through the localization of plasmin on the matured hepatocytes
Taiichiro Seki, Nobuaki Okumura, Kasumi Ishii, Atsushi Miura, Takashi Hosono
6:15 pm - 44 dot Characterization of a uPAR-LDLR-like protein interaction site and its impact on cell migration and angiogenesis
Clemens Pausz, Rula Mawas, Anastasia Chillá, Matthias Unseld, René Novotny, Gerald Prager
6:30 pm - 45 dot PAI-2 inhibits bacterial proteases
Jessica Neilands, Gunnel Svensäter, Bertil Kinnby
6:45 pm - 46 dot Involvement of both tPA and LRP-1 in regulating circadian clock phase
Joanna Cooper, Rebecca Prosser
7:00 pm - Outdoor Grill, Digital Visualization Theater, Tour of Jordan Hall
   
Saturday, June 8th, 2013

McKenna Hall
8:30 am - Oral Session 9 – In vivo Models of the Plasminogen System and Related Proteins in Regulating Biological and Pathobiological Responses (Chairs: Katherine Hajjar and Riku Das)
8:30 am - 47 dot LRP1 protects the vasculature by regulating protein levels of connective tissue growth factor and HtrA1 in the aorta
Selen C. Muratoglu, Shani Belgrave, Brian Hampton, Mary Migliorini, Ling Chen, Irina Mikhailenko, Dudley K. Strickland
8:45 am - 48 dot The urokinase plasminogen activator receptor (uPAR) regulates intestinal macrophage phagocytosis and polarization in experimental colitis
Marco Genua, Silvia D-Alessio, Javier Cibella, Alessandro Gandelli, Emanuela Sala, Carmen Correale, Vincenzo Arena, Alberto Malesci, Stefania Vetrano, Silvio Danese
9:00 am - 49 dot Understanding the mechanism of activated protein C inhibition by PAI-1 in the presence of vitronectin using an in vivo model system
Kamlesh Gupta, Deborah Donahue, Francis Castellino, Victoria Ploplis
9:15 am - 50 dot PAI-1 is a critical determinant of senescence and survival in klotho mice, a murine model of accelerated aging
Mesut Eren, Amanda Boe, Sheila B. Murphy, Aaron T. Place, Varun Nagpal, Luisa Morales-Nebreda, Daniela Urich, G. R. S. Budinger, Gökhan M. Mutlu, Toshio Miyata, Douglas E. Vaughan
9:30 am - 51 dot Real time imaging of plasminogen accumulation in platelet-rich micro-thrombus and its effective lysis by tPA infusion in vivo
Aki Tanaka, Yuko Suzuki, Tomasz Brzoska, Hideto Sano, Tetsumei Urano
9:45 am - 52 dot The annexin A2 system, a novel player in innate immune responses.
Katherine A. Hajjar, Elle Flood, Brian Scharf, Laura Santambrogio
10:00 am - Break
10:15 am - Oral Session 10 – Protease Systems and Receptors in Cancer (M. Sharon Stack and Marco Genua)
10:15 am - 53 dot Glycolipid anchored and soluble uPAR forms are independent prognostic markers in colorectal cancer
Martin Illemann, Tine Thurison, Ida Lund, Hans Jergen Nielsen, Ole Didrik Laerum, Ib Jarle Christensen, Gunilla HØyer-Hansen
10:30 am - 54 dot A novel uPAR targeted monoclonal antibody, ATN-658, binds to a previously unidentified epitope on uPAR and inhibits tumor growth and metastasis in vivo
Andrew P. Mazar, Irawati Kandela, Andrey Ugolkov, Giulio Francia, Shafaat Rabbani, Robert S. Kerbel, Xiang Xu, Cai Yuan, Mingdong Huang
10:45 am - 55 dot Activation of uPAR signaling in treated glioblastoma multiforme Jingjing Hu, Jill Wykosky, Webster K. Cavenee, Scott R. VandenBerg, Frank Furnari, Steven L. Gonias
11:00 am - 56 dot Chemical probing of the urokinase receptor in cancer metastasis
Samy Meroueh
11:15 am - 57 dot Matriptase promotes squamous cell carcinogenesis through a PAR-2-NFκB signaling axis
Katiuchia Sales, Stine Friis, Sine Godiksen, Joanne Konkel, Karina Hansen, Roman Szabo, Lotte Vogel, Wanjun Chen, Silvio J. Gutkind, Thomas Bugge
11:30 am - Closing Remarks – Francis J. Castellino
11:45 am - Box Lunch and Departure


Download the schedule in .pdf

Workshop Poster Session Lineup .pdf